+
A dry-fracture technique for the optimum preparation of microsporidia-infected tissues for scanning electron microscopy
стр.3-12
A technique for the optimum preparation of microsporidia for scanning electron microscopy is described. Microsporidian-infected tissues were subjected to a triple fixation procedure followed by slow dehydration and critical point drying. Fracturing of the tissue to reveal details of both host and parasite was delayed until immediately before coating of specimens with gold; the exposed parasites were therefore free of extraneous contamination, displayed minimum distortion and retained their spatial relationships with each other and with the host tissue. The value of the technique is illustrated by examination of microsporidia both with and without sporophorous vesicles, including species representing the genera Tuzetia, Amblyospora, Pleistophora and Nosema.
Загружаем данные из библиотечной системы...
Ключевые слова
+
Diversity of symbiotic consortia of prokaryotes in the cells of pelomyxids (Archamoeba, Pelomyxidae)
стр.13-25
Ludmila V. Chistyakova, Mariia A. Berdieva, Alexey Y. Kostygov, Alexander O. Frolov
The family Pelomyxidae includes archamoebae of the genera Mastigella and Pelomyxa. In contrast to other archamoebae, they always contain prokaryotic cytobionts in their cytoplasm. According to the morphological analysis of the cytobionts in 11 pelomyxid species, we classified them into 4 basic types, namely: “Af”, “Lt”, “S3” and “S4”. Being specific for each host species, these cytobionts form constant consortia which can be of two kinds: binary and ternary. Only “Af” cytobionts are common for all pelomyxids. These are slim rod-shaped Grampositive bacteria autofluorescing in UV. They have electron-dense cytoplasm and distinctive pointed cell ends. We found population of pelomyxae P. belevskii (Lt-) that have only “Af” cytobionts in the cytoplasm. Since these bacteria are found in all studied representatives of Pelomyxidae – both in Mastigella and Pelomyxa – this may indicate the early origin of such symbiosis, presumably associated with the separation of the group from the common stem of archamoebae. The presence of at least two shared cytobionts (“Af” and “Lt”) in the prokaryotic consortia of Mastigella and Pelomyxa serves as an additional evidence in favour of possible phylogenetic proximity of these two archamoebal groups.
Загружаем данные из библиотечной системы...
Ключевые слова
+
Culture collection of parasitic protists at the Zoological Institute RAS (CCPP ZIN RAS)
стр.26-42
Marina N. Malysheva, Maria A. Mamkaeva, Alexei Yu. Kostygov, Alexander O. Frolov, Sergey A. Karpov
Establishment of the bank of living cultures of trypanosomatids at the Laboratory of Protozoology, Zoological Institute of the Russian Academy of Sciences, St. Petersbug, started in 1979. The first edition of the collection catalogue (Malysheva et al., 2001) included 33 strains of trypanosomatids. The present, second edition contains descriptions of 52 strains of trypanosomatids and 28 strains of aphelids and parasitic chytridiomycetes. The strains of aphelids and parasitic chytridiomycetes were isolated and initially cultivated at the Center for Culturing Collection of Microorganisms of the Research park of St. Petersburg State University. This collection is named the Culture Collection of Parasitic Protists of the Zoological Institute RAS (CCPP ZIN RAS).
Загружаем данные из библиотечной системы...
Ключевые слова
