EXPERIMENTAL JUSTIFICATION OF NEW WAY OF PHARMACOLOGICAL CORRECTION FOR CONTACT FROSTBITE USING DSLET OPIOID PEPTIDE AND SEROTONIN ADIPINATE TO ENHANCE SURGYCAL TREATMENT
Chigunadze A.L., Artyushkova E.B., Mishustin V.N., Goryainova G.N., Artyushkova E.V.
The severity of complications and a large percentage of disability related to the occurrence cold injury lead to medical, social and economic problems. Considering the contact of cold injury as a variety of acute ischemia with the subsequent formation of early and late postischemic disorders as causes of disorders of blood circulation in the affected segments can be considered a disorder of the oxidant-antioxidant status and cytokine, systemic inflammation, reperfusion syndrome, the development of endothelial dysfunction, which ultimately leads to multiple organ failure. In the course of the study when assessing the microcirculatory changes caused by the simulation of frostbite, the most pronounced effect of the combined treatment of Serotonin adipinate 4.5 mg/kg and opioid peptide DSLET 10 μg/kg compared with monotherapy with the studied drugs was estimated. The most effective correction of indices of oxidant- antioxidant protection observed with the combination of necrectomy with a combination of Serotonin adipinate and opioid peptide DSLET, which was confirmed by approximating the indicators of malondialdehyde, superoxide dismutase, finite stable metabolites of nitrogen oxide, total antioxidant activity and catalase to the values of intact animals on the 14th day of the experiment and reducing the level of proinflammatory cytokines (interleukin-6, tumor necrosis factor-alpha) in 2 times (p < 0.05) compared to the Control group. The combined use of Serotonin adipinate 4.5 mg/kg and opioid peptide DSLET of 10 µg/kg in combination with active surgical tactics (necrectomy) helps reduce the severity and prevalence of alternative changes, accelerate the formation of granulation shaft, a more intense development of reparative processes in the simulation of cold injury compared with the monotherapy. The combined use of investigational drugs in combination with active surgical tactics led to the maximum enhancement of the survival of the animals with contact frostbite in the experiment.
PHARMACOKINETIC STUDIES DERIVED INDOLE SS-68 WITH ANTIARRHYTHMIC AND ANTIANGINAL PROPERTIES
Galenko-Yaroshevsky P. A., Kulikov A. L., Vinakov D.V., Avtina T.V., Suzdalev K.F., Pokrovskii M.V.
A method of quantitative determination of SS-68 derivative of indole in rabbit blood plasma by high performance liquid chromatography with tandem mass spectrometric detector (HPLC- MS/MS). Conducted pharmacokinetic studies SS-68 in the body of rabbits. Set the main pharmacokinetic parameters of the substance that allow you to optimize the future use of it’s as a potential drug in cardiology practice.
PHARMACOLOGICAL EFFICIENCY OF STATINS AND L-NORVALIN AT AN ENDOTOXIN-INDUCED ENDOTHELIAL DYSFUNCTION
Ivlitskaya I.L., Korokin M.V., Loktionov A.L.
In the animals with endotoxin-induced endothelial dysfunction there were imbalance of contractile response of vessels wall observes when carrying out functional tests, increase of coefficient of endothelial dysfunction, decrease in contractility of a myocardium, activation processes of peroxidation of lipids and decreased activity of antioxidant systems, level of stable metabolites of nitrogen oxide. On extent of decrease in corrective effects on indicators of functional activity of a vessel wall, contractility of a myocardium, oxidatic indicators and level of stable metabolites of nitrogen oxide are studied preparations settle down in the following sequence: L-norvalin and rozuvastatin (coefficient of endothelial dysfunction - 1,2±0,1) → L- norvalin and simvastatin (coefficient of endothelial dysfunction - 1,5±0,1) → rozuvastatin (coefficient of endothelial dysfunction - 1,6±0,1) → L-norvalin (coefficient of endothelial dysfunction - 1,7±0,2) → symvastatin (coefficient of endothelial dysfunction - 2,0±0,2).
METHOD OF CORRECTION OF ENDOTHELIAL DYSFUNCTION WITH COMBINATION OF ADEMETIONINE AND TAURINE
Khadieva T.A., Dovgan A. P., Pokroskaya T.G.
In the experiment, was made a simulation of endothelial dysfunction in male rats of Wistar-line byintraperitoneal administration of L-NAME at a dose of 25 mg/kg/day for 7 days. Deficiency of nitric oxide in result of the blockade of NO-synthase was accompanied by violation of endothelium-dependent and endothelium-undependentvasodilation assessed in pharmacological trials, which was reflected in the increase of the coefficient endotelialny dysfunction.In this case, for correction of endothelial dysfunction intraperitoneallyademetionine in the dose of 150 mg/kg and after an hour taurine at a dose of 260 mg/kg was injected the animal once a day for 7 days.The method provides effective impact of the combination of hepatoprotectorAdemetionine in the dose of 150 mg/kg/day and a sulfur-containing amino acid Taurine in the dose of 260 mg/kg/day on the functioning of the vascular endothelium, and has endotheliopathy effect on models L- NAME-induced deficiency of nitric oxide, which includesthe endothelium-dependent vasodilation and the decrease of coefficient of endothelial dysfunction.
MELATONIN ATTENUATES THE STRESS-INDUCED DISORDERS IN PARODONTIUM AND LIVER
Lyashev Y.D., Serikov V. S.
The results of investigation illustrate the lipid peroxidation activation in different tissues under the stress influence. The obtained results convince the different resistance of animals to stress. The decrease of free and bound oxyprolin content, as well as glycosaminoglycans, which are the main components of connective tissue of gingiva, after prolonged stress has been established. Stress causes the development of structural and functional disorders in the liver tissue. Melatonin is effective to inhibit the stress-induced changes in liver and parodontium. The higher efficiency of melatonin at the dose 1,0 mg/kg over 0,2 mg/kg was established. The obtained results open the prospects of the using of melatonin and its artificial analogues for pharmacological treatment of stress-induced disorders in parodontium and liver.
EXPERIMENTAL SUBSTANTIATION EFFICIENCY OF APPLICATION OF THE AMARANTH SEEDS OIL AT COMPLICATIONS INVOKED BY ISONIAZID
In an experimental research found that amaranth seeds press oil in dose of 600 mg/kg of the Isoniazid intoxication (in dose of 542 mg/kg during 6 days) prevents death of animals, reduces considerably an expressiveness of central and peripheral nervous system pathological changes, promotes normalization of digestive function and animals clinical state, lowers cytolysis syndrome manifestation degree promoting full normalization of aminotransferases activity on the 14th day. By means of biomicroscopy, using the original method of rats small intestine mesentery microvascular monitoring in an abdominal cavity, the peculiarities of microcirculatory processes disturbances invoked by high doses of Isoniazid, are revealed for the first time and the possibility of it correction via amaranth seeds oil.
CORRECTION OF ISCHEMIC DAMAGE TO THE RETINA ON APPLICATION OF PHARMACOLOGICAL PRECONDITIONING OF RECOMBINANT ERYTHROPOIETIN
To study the protective properties of pharmacological preconditioning with recombinant erythropoietin suberitro stimulating dose of 50 IU / kg in the retina of the eye, was the model of retinal ischemia, ischemia with the assessment of the temporary simulation mode of the retina of rats with the instrumental methods of analysis and morphometric studies. The most suitable model was a model 30 minute ischemia with subsequent reperfusion periods of 1 hour to 72 hours. We studied the protective effect preconditioning suberitro stimulating recombinant erythropoietin in a dose of 50 IU/kg, preconditioning distant ischemic and emoxipine 2 mg/kg per rat model of retinal ischemia. In the experiment, it was revealed that the recombinant erythropoietin (50 IU/kg) prevents the development of degenerative retinal layers due to ischemic damage more than distant ischemic preconditioning and emoxipine. The observed protective effect of erythropoietin us with the development of ischemia was confirmed by laser Doppler flowmetry, electroretinography and morphometry. Using suberitro stimulating recombinant erythropoietin in a dose of 50 IU/kg, 30 minutes prior to the pathology simulation eliminates erythropoietic effect, and the lack of positive dynamics in groups with preliminary administration of glibenclamide 5 mg/kg, the key role of ATP-dependent potassium channels in a preconditioning mechanism implementation. Identification and use of pharmacological agents, which have the effect of preconditioning, may be a new approach in the correction and prevention of retinal ischemia, which is the leading element in the pathogenesis of a number of visual pathologies. The possibility of pharmacological preconditioning with erythropoietin ischemic lesions of the retina is essential for the development of anti-ischemic agents for the treatment and prevention of ocular pathologies of ischemic origin.
DIABETIC FOOT SYNDROME: IMPORTANCE OF MICROBIOLOGICAL MONITORING AND ANTIMICROBIAL PENETRATION OF CHEMOTHERAPEUTIC AGENTS INTO THE SOFT TISSUE LOWER LIMB IN DETERMINING THE TREATMENT
Malorodova T.N., Pokrovskaya T.G., Kazakova E. E., Urojevskaya J. S.
Foot infections are a common and serious problem in persons with diabetes. Persistent ulcers in patients with diabetic foot syndrome in 85% of cases lead to amputation because of secondary infection and growing gangrene that significantly worsen the prognosis. The review provides the information on the spectrum of microorganisms, initiating the development of clinical and morphological forms of diabetic foot infections. Special attention has been paid to the choice in diabetic foot infections antibacterial medications based on indicators of sensitivity to them by allocated microorganisms and features of therapeutic concentrations formation in the skin, soft tissues or bone. Clinical data of researchers in the degree of penetration of various antibacterial agents in soft tissue and depend on severity lesions. In our review, it was shown that the most common pathogen was Staphylococcus aureus, followed by Pseudomonas aeruginosa, Enterobacteriaceae: E. coli, Proteus spp., Klebsiella pneumoniae, Enterobacter spp., and Acinetobacter spp. due to multidrug-resistant organisms, such as extended-spectrum beta- lactamase-producing Gram-negative rods or methicillin-resistant Staphylococcus aureus have emerged as substantial problem. Data on the frequency and the sensitivity of key pathogens in diabetic foot differ in Russian and foreign researchers, which causes the necessary treatment considering local peculiarities allocated pathogens and the degree of penetration of anti-infective agents in the affected tissues.
PHARMACOLOGICAL MANAGEMENT OF IMMUNE AND OXIDATIVE DISTURBANCES IN PATIENTS WITH ENCEPHALOPATHY ON THE BACKGROUND OF HYPERTENSION
Shulginova A.A., Voronina E.Yu., Bistrova N. A.
Prior to treatment in patients with discirculatory encephalopathy stage II affected by hypertensive disease set at the system level elevation of proinflammatory and regulatory cytokines, stable metabolites of nitric oxide, a decrease of immunoglobulins M, G, A, disbalance of the complement system components, development oxidative stress. Inclusion of a comprehensive drug treatment of patients with discirculatory encephalopathy cerebrolysin combination with meksidol normalizes the concentration of C3 and C4 components of the complement system, IgG, malondialdehyde, catalase activity, total antioxidant activity of blood serum, corrects, but not to the level of standards, the contents of cytokines (TNF, IL-1β, IL-6, IL- 8, IL-18, IFγ, IL-2, IL-17), complement component C5, IgA, acylhydrohyperoxide, neopterin and increases anti-inflammatory cytokines. Using emoxipine and piracetam as part of pharmacotherapy vascular encephalopathy stage II, compared with a combination of cerebrolysin and meksidol to further normalize levels of IL-17, C-reactive protein, increasingly closer to that of a control group of IL-6, IL-2 IL-18, C5, C5a component of complement, IgM, AGP, SMNO, increases the concentration of the complement system and cytokine network controllers. Included in the complex pharmacotherapy of patients with discirculatory encephalopathy and a combination aktovegin and cereton has maximum efficiency, normalizing 52% and 48% broken correlation immune parameters.
INFLUENCE OF THE ENTEROSORBENT ON QUALITY OF EGGS
Buhanov V.D., Vesentsev A.I., Naumenko L.I., Sokolovsky P.V., Pankova O.N.
In this article research the influence of natural montmorillonite containing sorbent used as a mineral supplement in animal feed in an amount of 30 g per 1 kg of sorbent feed on commercial quality table eggs at his dacha hens-layers. Found that feeding hens’ mineral feed additive reduces the toxic chemical elements in a chicken egg. It was found that the weight of the eggs in the experimental group compared to the control group, was significantly increased by 3,5% (p <0,05) after the completion of feeding the mineral sorbent. Despite the fact that in the control group, egg weight did not change and remained virtually unchanged. There is a bright pigmentation and reduction of "marbling" of the egg shell in the experimental birds. Set reduction "marbling" of 4.9 % and a decrease in light-colored eggs by 48.3 %. It has been established that chicken’s experimental group had a higher feed consumption and conversion (1.4 kg) as compared to control birds (1.3 kg). The best feed conversion per unit of egg products was observed in the experimental birds due to increased productivity. Proved that the marketability of the eggs is in direct proportion to the consumption of laying hens’ natural montmorillonite containing sorbent. Found that despite the great variety of indicators that characterize the quality of table eggs, the main thing is the maximum permissible levels of pollutants in them. Concluded that when administered in feed for laying hens 3 wt. % Montmorillonite clay containing eggs canbe obtained with low dietary content of harmful substances entering the body to feed the birds .
THE INFLUENCE OF ELECTROLYTES OF THE IONS AND THE INHIBITOR ON THE ACTIVITY OF TOTAL ATPASE OF ERYTHROCYTES OF BROILER CHICKENS
Mosyagina I.P., Mosyagin V.V.
The total ATPase activity of erythrocytes of broiler chickens depends on the concentration of ions Na+ and K+. The ATPase activity at 76.5% due to magnesium ions, 96.5% of the sodium ions and 47.6% of potassium ions. Maximum ATPase activity was observed in incubation medium containing ions: Na+ 120 mmol/ml, K+ 20 mmol/ml; Mg2+ - 3.0 mmol/ml and was of 9,240,23 nmol •mg protein-1•min-1. The ATPase activity has no effect of the specific inhibitor - strophanthin-K in the concentration range of 0-100 mg•l-1 in the medium containing ions Na+ and K+, and in an environment without them.
A METHOD FOR PRODUCTION OF PHYTOMINERALSORBENT, PHYSICAL AND CHEMICAL PROPERTIES OF IT, EFFECT ON THE LIVING SYSTEMS AND THE QUALITY OF THE LIVESTOCK INDUSTRY PRODUCTS
Yakovleva I.N., Shaposhnikov A.A., Vesenzev A.I., Kovaleva V. U., Zakirova L. R., Shevchenko T.S., Shentseva E.A.
Numerous experimental studies have shown that the sorption-active substances can clarify the reservoir, soils, drinking municipal water from unwanted impurities with a sufficient degree of effectiveness; eliminate the potentially toxic for health chemicals of different chemical nature from human body. This is ensuring their using in the animal production practice, veterinary medicine and medicine. The main purpose and focus of this study are associated with the solution of problems of the maintaining physiological and biochemical status of live-stock animals. It provides their productivity and biological value of obtained products.